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Western Blot (Transfected lysate)

CIITA polyclonal antibody (Cat # PAB9942), generated by immunization with bacterially produced FLAG-CIITA aa 1-333, was tested by immunoblot against lysates of COS-7 cells after transient transfection, separately, with pcDNA3-FLAG-CIITA-336-884 and pcDNA3-HA-CIITA.
In panel A, both samples on PVDF were incubated with 10 ug/mL mouse anti-FLAG antibody (Sigma) for 45'.
This blot demonstrates that FLAG-CIITA-336-842 was successfully over-expressed in the Cos-7 cells.
In panel B, both samples on PVDF were incubated with a 1 : 500 dilution of CIITA polyclonal antibody (Cat # PAB9942) for 45'.
The lane containing the lysate of pcDNA3-HA-CIITA transfected cells contains a single band at ~130 kD molecular weight, whereas the lane containing lysate from pcDNA3-FLAG-CIITA-336-842 transfected cells shows no reactivity.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

4261

Gene Name

CIITA

Gene Alias

C2TA, CIITAIV, MHC2TA, NLRA

Gene Description

class II, major histocompatibility complex, transactivator

Omim ID

126200 180300 209920 600005

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. The protein also binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. [provided by RefSeq

Other Designations

MHC class II transactivator type III|NLR family, acid domain containing|class II transactivator|nucleotide-binding oligomerization domain, leucine rich repeat and acid domain containing