CDC2 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDC2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CDC2.
Host
Rabbit
Theoretical MW (kDa)
34
Reactivity
Human, Mouse, Rat
Specificity
Antiserum will specifically react with a 34 KDa cdc2 protein from human, rat and mouse tissue. Cross reactivity with cdc2 from other species may also occur.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:500-1:2000)
Western Blot (1:500-1:1000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
CDC2 polyclonal antibody (Cat # PAB9939) was used todetect human CDC2 by western blot in untreated (Contol) and drug treated lysates of MCF-7 cells.
Lane 1-4 represents 3.1 uM, 6.2 uM, 12.5 uM and 25.0 uM genistein treatment of cells before lysates were prepared. Detection occurs using a 1 : 1,000 dilution.
Although this antiserum detects non-specific bands at higher MW, a clear induction ofsignal is observed as the concentration of drug is increased.
Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — CDC2
Entrez GeneID
983Gene Name
CDC2
Gene Alias
CDC28A, CDK1, DKFZp686L20222, MGC111195
Gene Description
cell division cycle 2, G1 to S and G2 to M
Omim ID
116940Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019660|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2 protein|cyclin-dependent kinase 1|p34 protein kinase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.
Giordano A, Whyte P, Harlow E, Franza BR Jr, Beach D, Draetta G.
Cell 1989 Sep; 58(5):981.
Application:IP, Human, HeLa cells.
-
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
Draetta G, Beach D.
Cell 1988 Jul; 54(1):17.
Application:AFC, IP, KA, WB-Ce, WB-Re, Human, HeLa cells, Recombinant proteins.
-
A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com