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Last updated: 2014/8/25
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AKT1 polyclonal antibody

  • Catalog # : PAB9932
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  • Specification
  • Product Description:
  • Sheep polyclonal antibody raised against synthetic peptide of AKT1.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human AKT1.
  • Host:
  • Sheep
  • Reactivity:
  • Chicken, Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.1% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using AKT1 polyclonal antibody (Cat # PAB9932) shows detection of endogenous protein in whole cell extracts from H2O2 treated MCF-7 cells (Lane 2).
    Recombinant AKT1 is detected in lane 3.
    Lanes 1 and 4 contain wcl from HeLa and A-431 cells, respectively.
    Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown).
    The band at ~55-60 KDa, indicated by the arrowhead, corresponds to the expected molecular weight of AKT1.
    Primary antibody was diluted 1 : 300 in 1% BLOTTO and reacted with the membrane overnight at 4°C.
    IRDYE800™ conjugated Dnky-a-Sheep IgG was used at a 1 : 10,000 dilution for 45 min at room temperature.
  • ELISA
  • Application Image
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 207
  • Protein Accession#:
  • NP_005154;P31749
  • Gene Name:
  • AKT1
  • Gene Alias:
  • AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
  • Gene Description:
  • v-akt murine thymoma viral oncogene homolog 1
  • Gene Summary:
  • The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
  • Other Designations:
  • RAC-alpha serine/threonine-protein kinase,murine thymoma viral (v-akt) oncogene homolog-1,protein kinase B,rac protein kinase alpha
  • Interactome
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