AKT1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Immunoblotting of AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 500 dilution to detect AKT1 by Western blot.
Approximately 20 ug of an NIH/3T3 whole cell lysate was loaded on a 10% NuPage SDS-PAGE gel using MOPS buffer.
After washing, a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Pierce Chemical's Super Signal™ substrate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 1,000 dilution to detect AKT1 by immunohistochemistry. In panel (A) normal colon tissue is stained. Panel (B) shows tumor tissue. Tissue was formalin-fixed and paraffin embedded.
Immunofluorescence
Immunofluorescence microscopy of AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT1 construct. AKT1 polyclonal antibody (Cat # PAB9931) staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKT1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 460-480 of AKT1.
Sequence
CRPHFPQFSYSASGTA
Host
Rabbit
Reactivity
Chicken, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:500-1:2000)
Immunofluorescence (1:100-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.1% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Immunoblotting of AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 500 dilution to detect AKT1 by Western blot.
Approximately 20 ug of an NIH/3T3 whole cell lysate was loaded on a 10% NuPage SDS-PAGE gel using MOPS buffer.
After washing, a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Pierce Chemical's Super Signal™ substrate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 1,000 dilution to detect AKT1 by immunohistochemistry. In panel (A) normal colon tissue is stained. Panel (B) shows tumor tissue. Tissue was formalin-fixed and paraffin embedded.Immunofluorescence
Immunofluorescence microscopy of AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT1 construct. AKT1 polyclonal antibody (Cat # PAB9931) staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?
Lawlor MA, Alessi DR.
Journal of Cell Science 2001 Aug; 114(Pt 16):2903.
Application：IHC, WB-Tr, Human, Cancers, Mammalian cells.
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Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture.
Alessi DR.
Biochemical Society Transactions 2001 May; 29(Pt 2):1.
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PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?
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