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Last updated: 2014/12/14
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AKT1 polyclonal antibody

  • Catalog # : PAB9931
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of AKT1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 460-480 of AKT1.
  • Sequence:
  • CRPHFPQFSYSASGTA
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:500-1:2000)
    Immunofluorescence (1:100-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.1% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblotting of AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 500 dilution to detect AKT1 by Western blot.
    Approximately 20 ug of an NIH/3T3 whole cell lysate was loaded on a 10% NuPage SDS-PAGE gel using MOPS buffer.
    After washing, a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Pierce Chemical's Super Signal™ substrate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 1,000 dilution to detect AKT1 by immunohistochemistry. In panel (A) normal colon tissue is stained. Panel (B) shows tumor tissue. Tissue was formalin-fixed and paraffin embedded.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence microscopy of AKT1 polyclonal antibody (Cat # PAB9931) was used at a 1 : 80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT1 construct. AKT1 polyclonal antibody (Cat # PAB9931) staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
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