ABCB6 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using ABCB6 polyclonal antibody (Cat # PAB9926) (right panel, lane B6) shows detectionof Flag™-tagged human ABCB6 proteinat 104 KDa and a truncated form of theprotein at 79 KDa (arrowheads).
The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein.
A lysate prepared from KB cells without a vector insert (CV lane) should no reactivity with the antibody.
The left panel shows a similar staining pattern using an anti-Flag™ antibody asa positive control.
The membrane was probed with the primary antibody dilutedto 1 : 1,400.
Personal Communication, Jill Paterson, CCR-NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ABCB6.
Immunogen
A synthetic peptide corresponding to amino acids 440-45 of human ABCB6.
Host
Rabbit
Reactivity
Dog, Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:40000-1:200000)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using ABCB6 polyclonal antibody (Cat # PAB9926) (right panel, lane B6) shows detectionof Flag™-tagged human ABCB6 proteinat 104 KDa and a truncated form of theprotein at 79 KDa (arrowheads).
The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein.
A lysate prepared from KB cells without a vector insert (CV lane) should no reactivity with the antibody.
The left panel shows a similar staining pattern using an anti-Flag™ antibody asa positive control.
The membrane was probed with the primary antibody dilutedto 1 : 1,400.
Personal Communication, Jill Paterson, CCR-NCI, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ABCB6
Entrez GeneID
10058Protein Accession#
Q9NP58Gene Name
ABCB6
Gene Alias
ABC, ABC14, EST45597, FLJ22414, MTABC3, PRP, umat
Gene Description
ATP-binding cassette, sub-family B (MDR/TAP), member 6
Omim ID
605452Gene Ontology
HyperlinkGene Summary
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This half-transporter likely plays a role in mitochondrial function. Localized to 2q26, this gene is considered a candidate gene for lethal neonatal metabolic syndrome, a disorder of mitochondrial function. [provided by RefSeq
Other Designations
ATP-binding cassette half-transporter|ATP-binding cassette, sub-family B, member 6
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Interactome
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Pathway
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Disease
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Publication Reference
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MTABC3, a novel mitochondrial ATP-binding cassette protein involved in iron homeostasis.
Mitsuhashi N, Miki T, Senbongi H, Yokoi N, Yano H, Miyazaki M, Nakajima N, Iwanaga T, Yokoyama Y, Shibata T, Seino S.
The Journal of Biological Chemistry 2000 Jun; 275(23):17536.
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MTABC3, a novel mitochondrial ATP-binding cassette protein involved in iron homeostasis.
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