EPHB1 polyclonal antibody
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More Files
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Immunoprecipitation
Immunoprecipitated was carried out with EPHB1 (CY) polyclonal antibody on CHO cells that was transfected with pSRalpha-huEphB1/HA, driving expression of HA epitope tagged EphB2. Western blot analysis were then carried out with anti-HA epitope.
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Specification
Product Description
Sheep polyclonal antibody raised against partial recombinant EPHB1.
Immunogen
Recombinant GST fusion protein corresponding to amino acids 586-984 of human EPHB1.
Host
Sheep
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1-10 ug/mL)
Immunoprecipitation (10-20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Immunoprecipitated was carried out with EPHB1 (CY) polyclonal antibody on CHO cells that was transfected with pSRalpha-huEphB1/HA, driving expression of HA epitope tagged EphB2. Western blot analysis were then carried out with anti-HA epitope. -
Gene Info — EPHB1
Entrez GeneID
2047Gene Name
EPHB1
Gene Alias
ELK, EPHT2, FLJ37986, Hek6, NET
Gene Description
EPH receptor B1
Omim ID
600600Gene Ontology
HyperlinkGene Summary
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq
Other Designations
eph tyrosine kinase 2|ephrin receptor EphB1|soluble EPHB1 variant 1
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Interactome
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