14-3-3 (phospho S58) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of 14-3-3 protein.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S58 of rat 14-3-3 protein.
Host
Rabbit
Theoretical MW (kDa)
29
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Sheep, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat brainstem lysate showing specific immuno- labeling of the ~29k 14-3-3 protein phosphorylated at Ser58 (Control, lane 1). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep, lane 2) but not by the corresponding dephosphopeptide (not shown).Immunofluorescence
Immunostaining of mouse hippocampal cryosections with 14-3-3 (phospho S58) polyclonal antibody (Cat # PAB9657) (green). GFAP positive astrocytes (red) and DAPI labeled nuclei (blue). Photo courtesty of Robert Wine. -
Publication Reference
-
14-3-3 proteins: a number of functions for a numbered protein.
Bridges D, Moorhead GB.
Science's STKE : Signal Transduction Knowledge Environment 2005 Aug; 2005(296):re10.
Application:WB, Human, Human mammalian cells .
-
14-3-3 protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding.
Silhan J, Obsilova V, Vecer J, Herman P, Sulc M, Teisinger J, Obsil T.
The Journal of Biological Chemistry 2004 Nov; 279(47):49113.
-
Unlocking the code of 14-3-3.
Dougherty MK, Morrison DK.
Journal of Cell Science 2004 Apr; 117(Pt 10):1875.
-
14-3-3 proteins: a number of functions for a numbered protein.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com