Aqp2 (phospho S261) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the Aqp2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown).
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Aqp2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S261 of rat Aqp2.
Host
Rabbit
Theoretical MW (kDa)
29, 37
Reactivity
Bovine, Chicken, Dog, Human, Mouse, Primates, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C with 50% glycerol. Store at -80°C for long term storage.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the Aqp2 protein phosphorylated at Ser261. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide) but not by the corresponding dephospho-peptide (not shown). -
Gene Info — Aqp2
-
Publication Reference
-
Double Knockout of Carbonic Anhydrase II (CAII) and Na+-Cl- Cotransporter (NCC) Causes Salt Wasting and Volume Depletion.
Xu J, Barone S, Brooks MB, Soleimani M.
Cellular Physiology and Biochemistry 2013 Dec; 32(7):173.
Application：WB, Mouse, Kidney.
-
Bardet-Biedl syndrome highlights the major role of the primary cilium in efficient water reabsorption.
Marion V, Schlicht D, Mockel A, Caillard S, Imhoff O, Stoetzel C, van Dijk P, Brandt C, Moulin B, Dollfus H.
Kidney International 2011 May; 79(9):1013.
Application：WB, Human, HCD cells.
-
Characterization of two novel missense mutations in the AQP2 gene causing nephrogenic diabetes insipidus.
Iolascon A, Aglio V, Tamma G, D'Apolito M, Addabbo F, Procino G, Simonetti MC, Montini G, Gesualdo L, Debler EW, Svelto M, Valenti G.
Nephron. Physiology 2007 Dec; 105(3):p33.
-
Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct.
Hoffert JD, Nielsen J, Yu MJ, Pisitkun T, Schleicher SM, Nielsen S, Knepper MA.
American Journal of Physiology. Renal Physiology 2007 Feb; 292(2):F691.
Application：Dot, IF, IHC-P, Rat, Rat kidney, Recombinant protein.
-
Volume regulation in cortical collecting duct cells: role of AQP2.
Ford P, Rivarola V, Chara O, Blot-Chabaud M, Cluzeaud F, Farman N, Parisi M, Capurro C.
Biology of the Cell 2005 Sep; 97(9):687.
-
Double Knockout of Carbonic Anhydrase II (CAII) and Na+-Cl- Cotransporter (NCC) Causes Salt Wasting and Volume Depletion.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com