AKT3 (phospho S472) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT3.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S472 of human AKT3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Peptide affinity purification
Recommend Usage
ELISA (1:1000)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot blot analysis of AKT3 (phospho S472) polyclonal antibody (Cat # PAB8141) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL. -
Gene Info — AKT3
Entrez GeneID
10000Protein Accession#
NP_005456;Q9Y243Gene Name
AKT3
Gene Alias
DKFZp434N0250, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2
Gene Description
v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Omim ID
611223Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000037911|OTTHUMP00000037912|RAC-gamma serine/threonine protein kinase|protein kinase B gamma|serine threonine protein kinase, Akt-3|v-akt murine thymoma viral oncogene homolog 3
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Interactome
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Pathway
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Disease
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Publication Reference
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Transforming growth factor-beta1 stimulated protein kinase B serine-473 and focal adhesion kinase tyrosine phosphorylation dependent on cell adhesion in human hepatocellular carcinoma SMMC-7721 cells.
Xu Z, Ma DZ, Wang LY, Su JM, Zha XL.
Biochemical and Biophysical Research Communications 2003 Dec; 312(2):388.
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Defective signaling through Akt-2 and -3 but not Akt-1 in insulin-resistant human skeletal muscle: potential role in insulin resistance.
Brozinick JT Jr, Roberts BR, Dohm GL.
Diabetes 2003 Apr; 52(4):935.
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Gene expression profiling in prostate cancer cells with Akt activation reveals Fra-1 as an Akt-inducible gene.
Tiwari G, Sakaue H, Pollack JR, Roth RA.
Molecular Cancer Research: MCR 2003 Apr; 1(6):475.
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Transforming growth factor-beta1 stimulated protein kinase B serine-473 and focal adhesion kinase tyrosine phosphorylation dependent on cell adhesion in human hepatocellular carcinoma SMMC-7721 cells.
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