CD40LG polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of rat spleen tissue lysate. Using CD40LG polyclonal antibody (Cat # PAB7962) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embbed human mammary cancer section. Staining CD40LG in cytoplasm DBA chromogenic reaction with CD40LG polyclonal antibody (Cat # PAB7962, 1 ug/mL).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CD40LG.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human CD40LG.
Host
Rabbit
Theoretical MW (kDa)
29.3
Reactivity
Human, Mouse, Rat
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of rat spleen tissue lysate. Using CD40LG polyclonal antibody (Cat # PAB7962) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embbed human mammary cancer section. Staining CD40LG in cytoplasm DBA chromogenic reaction with CD40LG polyclonal antibody (Cat # PAB7962, 1 ug/mL). -
Gene Info — CD40LG
Entrez GeneID
959Gene Name
CD40LG
Gene Alias
CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Gene Description
CD40 ligand
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq
Other Designations
CD40 antigen ligand|OTTHUMP00000024130|T-B cell-activating molecule|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5
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Interactome
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Pathway
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Disease
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Publication Reference
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Preprocedural level of soluble CD40L is predictive of enhanced inflammatory response and restenosis after coronary angioplasty.
Cipollone F, Ferri C, Desideri G, Paloscia L, Materazzo G, Mascellanti M, Fazia M, Iezzi A, Cuccurullo C, Pini B, Bucci M, Santucci A, Cuccurullo F, Mezzetti A.
Circulation 2003 Dec; 108(22):2776.
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CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome.
Allen RC, Armitage RJ, Conley ME, Rosenblatt H, Jenkins NA, Copeland NG, Bedell MA, Edelhoff S, Disteche CM, Simoneaux DK, et al..
Science 1993 Feb; 259(5097):990.
Application：WB-Tr, Human, 293 cells.
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Preprocedural level of soluble CD40L is predictive of enhanced inflammatory response and restenosis after coronary angioplasty.
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