PTPN6 (phospho Y536) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min (lanes 1 & 2). The blot was exposed to alkaline phosphatase (lane 2) then probed with PTPN6 (phospho Y536) polyclonal antibody (Cat # PAB7923).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PTPN6.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y536 of human PTPN6.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
The sequence is highly conserved in rat and mouse SHP1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min (lanes 1 & 2). The blot was exposed to alkaline phosphatase (lane 2) then probed with PTPN6 (phospho Y536) polyclonal antibody (Cat # PAB7923).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PTPN6
Entrez GeneID
5777Gene Name
PTPN6
Gene Alias
HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1
Gene Description
protein tyrosine phosphatase, non-receptor type 6
Omim ID
176883Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
Other Designations
hematopoietic cell phosphatase|hematopoietic cell protein-tyrosine phosphatase|protein-tyrosine phosphatase 1C
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Interactome
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Pathway
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Disease
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Publication Reference
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Regulation of SHP-1 tyrosine phosphatase in human platelets by serine phosphorylation at its C terminus.
Jones ML, Craik JD, Gibbins JM, Poole AW.
The Journal of Biological Chemistry 2004 Sep; 279(39):40475.
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The role of C-terminal tyrosine phosphorylation in the regulation of SHP-1 explored via expressed protein ligation.
Zhang Z, Shen K, Lu W, Cole PA.
The Journal of Biological Chemistry 2003 Feb; 278(7):4668.
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Roles of the SHP-1 tyrosine phosphatase in the negative regulation of cell signalling.
Zhang J, Somani AK, Siminovitch KA.
Seminars in Immunology 2000 Aug; 12(4):361.
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Regulation of SHP-1 tyrosine phosphatase in human platelets by serine phosphorylation at its C terminus.
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