AKT1 (phospho T34) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A-431 cells, serum starved overnight (20 ug/lane 1) and calyculin A (100 nM) treated for 30 minutes (20 ug/lane 2). The blot was probed with AKT1 (phospho T34) polyclonal antibody (Cat # PAB7912).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T34 of human AKT1.
Host
Rabbit
Reactivity
Human, Rabbit, Rat
Specificity
This sequence is highly conserved in rat and mouse Akt.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-431 cells, serum starved overnight (20 ug/lane 1) and calyculin A (100 nM) treated for 30 minutes (20 ug/lane 2). The blot was probed with AKT1 (phospho T34) polyclonal antibody (Cat # PAB7912).Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT1
Entrez GeneID
207Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.
Powell DJ, Hajduch E, Kular G, Hundal HS.
Molecular and Cellular Biology 2003 Nov; 23(21):7794.
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PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond.
Marte BM, Downward J.
Trends in Biochemical Sciences 1997 Sep; 22(9):355.
Application：WB-Ce, WB-Tr, Human, Cancers, Mammalian cells.
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Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily.
Jones PF, Jakubowicz T, Pitossi FJ, Maurer F, Hemmings BA.
PNAS 1991 May; 88(10):4171.
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Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.
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