LIMK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot of activated mouse recombinant LIMK1untreated (lane 1) or treated with lambda phosphatase (lane 2). The blots were probed with LIMK1 polyclonal antibody (Cat # PAB7894).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LIMK1.
Immunogen
A synthetic peptide corresponding to C-terminus of human LIMK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence is conserved in rat and mouse LIMK1, and is not found in LIMK2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot of activated mouse recombinant LIMK1untreated (lane 1) or treated with lambda phosphatase (lane 2). The blots were probed with LIMK1 polyclonal antibody (Cat # PAB7894).Enzyme-linked Immunoabsorbent Assay
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Gene Info — LIMK1
Entrez GeneID
3984Protein Accession#
P53667Gene Name
LIMK1
Gene Alias
LIMK
Gene Description
LIM domain kinase 1
Omim ID
601329Gene Ontology
HyperlinkGene Summary
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. [provided by RefSeq
Other Designations
LIM motif-containing protein kinase|OTTHUMP00000025066
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Interactome
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Pathway
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Disease
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Publication Reference
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MAPKAPK-2-mediated LIM-kinase activation is critical for VEGF-induced actin remodeling and cell migration.
Kobayashi M, Nishita M, Mishima T, Ohashi K, Mizuno K.
The EMBO Journal 2006 Feb; 25(4):713.
Application：IP, WB, Human, MSS31 cells, HUVECs.
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Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics.
Edwards DC, Sanders LC, Bokoch GM, Gill GN.
Nature Cell Biology 1999 Sep; 1(5):253.
Application：IF, IP-WB, WB-Tr, Human, Mouse, HEK 293, BHK cells.
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Identification and characterization of a novel family of serine/threonine kinases containing two N-terminal LIM motifs.
Okano I, Hiraoka J, Otera H, Nunoue K, Ohashi K, Iwashita S, Hirai M, Mizuno K.
The Journal of Biological Chemistry 1995 Dec; 270(52):31321.
Application：IP-WB, Human, Monkey, A-431, COS-7 cells.
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MAPKAPK-2-mediated LIM-kinase activation is critical for VEGF-induced actin remodeling and cell migration.
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