TRIM2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
TRIM2 polyclonal antibody (Cat # PAB7498) (0.5 ug/mL) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TRIM2 polyclonal antibody (Cat # PAB7498)(6.3 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TRIM2.
Immunogen
A synthetic peptide corresponding to C-terminus of human TRIM2.
Sequence
DSGNHCFKVYRYLQ
Host
Goat
Theoretical MW (kDa)
81.5, 84.5, 83.3
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western blot (0.5-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (6-12 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TRIM2 polyclonal antibody (Cat # PAB7498) (0.5 ug/mL) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TRIM2 polyclonal antibody (Cat # PAB7498)(6.3 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRIM2
Entrez GeneID
23321Protein Accession#
NP_056086.2;NP_001123539.1Gene Name
TRIM2
Gene Alias
KIAA0517, RNF86
Gene Description
tripartite motif-containing 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified. [provided by RefSeq
Other Designations
tripartite motif protein 2|tripartite motif protein TRIM2
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Interactome
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Disease
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Publication Reference
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The tripartite motif family identifies cell compartments.
Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A.
The EMBO Journal 2001 May; 20(9):2140.
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The tripartite motif family identifies cell compartments.
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