GOT1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
GOT1 polyclonal antibody (Cat # PAB7415) (0.01 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GOT1 polyclonal antibody (Cat # PAB7415, 2.5 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GOT1.
Immunogen
A synthetic peptide corresponding to amino acids 157-167 of human GOT1.
Sequence
C-RSYRYWDAEKR
Host
Goat
Theoretical MW (kDa)
46.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western blot (0.01-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GOT1 polyclonal antibody (Cat # PAB7415) (0.01 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GOT1 polyclonal antibody (Cat # PAB7415, 2.5 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GOT1
Entrez GeneID
2805Protein Accession#
NP_002070.1Gene Name
GOT1
Gene Alias
GIG18
Gene Description
glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Omim ID
138180Gene Ontology
HyperlinkGene Summary
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq
Other Designations
OTTHUMP00000020254|aspartate aminotransferase 1|growth-inhibiting protein 18
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Interactome
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Pathway
- Alanine
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Carbon fixation in photosynthetic organisms
- Cysteine and methionine metabolism
- Isoquinoline alkaloid biosynthesis
- Metabolic pathways
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Publication Reference
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From the liver to the brain across the blood-brain barrier.
Teichberg VI.
PNAS 2007 Apr; 104(18):7315.
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From the liver to the brain across the blood-brain barrier.
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