Products

Western Blot (Tissue lysate)

WISP1 polyclonal antibody (Cat # PAB7404) (0.1 ug/mL) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Western Blot (Cell lysate)

WISP1 polyclonal antibody (Cat # PAB7404) (0.3 ug/mL) staining of Caco-2 cell lysate 1 (1) and Caco-2 cell lysate 2 (2) + peptide (3). (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence

WISP1 polyclonal antibody (Cat # PAB7404) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunofluorescence

WISP1 polyclonal antibody (Cat # PAB7404) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

WISP1 polyclonal antibody (Cat # PAB7404) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Entrez GeneID

8840

Protein Accession#

NP_003873.1;NP_543028.1

Gene Name

WISP1

Gene Alias

CCN4, WISP1c, WISP1i, WISP1tc

Gene Description

WNT1 inducible signaling pathway protein 1

Omim ID

603398

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq

Other Designations

WNT1 induced secreted protein 1|Wnt-1 inducible signaling pathway protein 1|wnt-1 signaling pathway protein 1