Sall4 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of Sall4.
Immunogen
A synthetic peptide corresponding to mouse Sall4.
Sequence
C-DQSKKGKGKPQN
Host
Goat
Theoretical MW (kDa)
113, 66.2
Reactivity
Rat
Specificity
This antibody is expected to recognize isoform a (NP_780512.2) and isoform b (NP_958797.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The Sall4 polyclonal antibody (Cat # PAB7278) (0.1 ug/mL) staining of rat testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Sall4
Entrez GeneID
99377Protein Accession#
NP_780512.2;NP_958797.2;NP_958798.2Gene Name
Sall4
Gene Alias
5730441M18Rik, AA407717, AL022809, AW536104, C330011P20Rik, C78083, C78563, Tex20
Gene Description
sal-like 4 (Drosophila)
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000017238|OTTMUSP00000017239|OTTMUSP00000017241|sal-like 4
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Publication Reference
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A protein interaction network for pluripotency of embryonic stem cells.
Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH.
Nature 2006 Nov; 444(7117):364.
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A protein interaction network for pluripotency of embryonic stem cells.
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