ACYP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ACYP1 polyclonal antibody (Cat # PAB7186) (0.01 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ACYP1.
Immunogen
A synthetic peptide corresponding to human ACYP1.
Sequence
C-PKSHIDKANFNNEK
Host
Goat
Theoretical MW (kDa)
11.3, 6.1
Reactivity
Human
Specificity
This antibody is expected to recognize isoform a (NP_001098.1) only.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ACYP1 polyclonal antibody (Cat # PAB7186) (0.01 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACYP1
Entrez GeneID
97Protein Accession#
NP_001098.1Gene Name
ACYP1
Gene Alias
ACYPE
Gene Description
acylphosphatase 1, erythrocyte (common) type
Omim ID
600875Gene Ontology
HyperlinkGene Summary
Acylphosphatase is a small cytosolic enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase, on the basis of their tissue localization. This gene encodes the erythrocyte acylphosphatase isoenzyme. Alternatively spliced transcript variants that encode different proteins were identified through data analysis. [provided by RefSeq
Other Designations
erythrocyte acylphosphatase 1
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Interactome
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Pathway
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Publication Reference
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A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase.
Paoli P, Pazzagli L, Giannoni E, Caselli A, Manao G, Camici G, Ramponi G.
The Journal of Biological Chemistry 2003 Jan; 278(1):194.
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A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase.
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