NEDD1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NEDD1.
Immunogen
A synthetic peptide corresponding to human NEDD1.
Sequence
C-NQTRNSEKFEKPEN
Host
Goat
Theoretical MW (kDa)
72
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NEDD1 polyclonal antibody (Cat # PAB7170) (0.1 ug/mL) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NEDD1
Entrez GeneID
121441Protein Accession#
NP_690869.1Gene Name
NEDD1
Gene Alias
FLJ35902, GCP-WD, TUBGCP7
Gene Description
neural precursor cell expressed, developmentally down-regulated 1
Omim ID
600372Gene Ontology
HyperlinkGene Summary
O
Other Designations
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Interactome
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Publication Reference
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NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly.
Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A.
The Journal of Cell Biology 2006 Feb; 172(4):505.
Application:IF, IP, WB, Human, HeLa cells.
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NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly.
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