HSPA8 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
HSPA8 polyclonal antibody (Cat # PAB7055) (2 ug/mL) staining of A-431 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of HSPA8.
Immunogen
A synthetic peptide corresponding to human HSPA8.
Sequence
C-EKLQGKINDEDKQK
Host
Goat
Theoretical MW (kDa)
70.9
Reactivity
Human
Specificity
This antibody is expected to recognize isoform 1 (NP_006588.1) only.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
HSPA8 polyclonal antibody (Cat # PAB7055) (2 ug/mL) staining of A-431 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSPA8
Entrez GeneID
3312Protein Accession#
NP_006588.1Gene Name
HSPA8
Gene Alias
HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP1, MGC131511, MGC29929, NIP71
Gene Description
heat shock 70kDa protein 8
Omim ID
600816Gene Ontology
HyperlinkGene Summary
The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq
Other Designations
LPS-associated protein 1|N-myristoyltransferase inhibitor protein 71|constitutive heat shock protein 70|heat shock 70kD protein 8|heat shock 70kd protein 10|heat shock cognate protein 54|heat shock cognate protein, 71-kDa|lipopolysaccharide-associated pro
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Interactome
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Pathway
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Disease
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Publication Reference
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Comparison of gene expression profile between human chondrons and chondrocytes: a cDNA microarray study.
Zhang Z, Fan J, Becker KG, Graff RD, Lee GM, Francomano CA.
Osteoarthritis and Cartilage 2006 May; 14(5):449.
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Comparison of gene expression profile between human chondrons and chondrocytes: a cDNA microarray study.
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