MAP3K7 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human MAP3K7 with DYKDDDDK tag probed with MAP3K7 polyclonal antibody (Cat # PAB7038) (1 ug/mL) in Lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in lane 3. Mock-transfected HEK293 probed with MAP3K7 polyclonal antibody (Cat # PAB7038) (1 mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MAP3K7.
Immunogen
A synthetic peptide corresponding to internal region of human MAP3K7.
Sequence
C-AELDQDEKDQQNT
Host
Goat
Reactivity
Human
Specificity
This antibody is expected to recognise isoform A (NP_003179.1) and isoform B (NP_663304.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human MAP3K7 with DYKDDDDK tag probed with MAP3K7 polyclonal antibody (Cat # PAB7038) (1 ug/mL) in Lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in lane 3. Mock-transfected HEK293 probed with MAP3K7 polyclonal antibody (Cat # PAB7038) (1 mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence. -
Gene Info — MAP3K7
Entrez GeneID
6885Protein Accession#
NP_003179.1;NP_663304.1Gene Name
MAP3K7
Gene Alias
TAK1, TGF1a
Gene Description
mitogen-activated protein kinase kinase kinase 7
Omim ID
602614Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
OTTHUMP00000016870|OTTHUMP00000016871|OTTHUMP00000016872|OTTHUMP00000016873|TGF-beta activated kinase 1|transforming growth factor-beta-activated kinase 1
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Interactome
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Pathway
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Disease
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Publication Reference
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TAK1 is a master regulator of epidermal homeostasis involving skin inflammation and apoptosis.
Emily Omori, Kunihiro Matsumoto, Hideki Sanjo, Shintaro Sato, Shizuo Akira, Robert C Smart, Jun Ninomiya-Tsuji.
The Journal of Biological Chemistry 2006 Jul; 281(28):19610.
Application：IF, IHC-Fr, IHC-P, WB-Tr, Mouse, Mouse dermal fibroblasts, Mouse keratinocytes, Mouse skin.
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TAK1 is a master regulator of epidermal homeostasis involving skin inflammation and apoptosis.
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