WNT3 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
WNT3 polyclonal antibody (Cat # PAB7032) (1 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
WNT3 polyclonal antibody (Cat # PAB7032, 2 ug/mL) staining of paraffin embedded human breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of WNT3.
Immunogen
A synthetic peptide corresponding to internal region of human WNT3.
Sequence
CGRGHNTRTEKRKEK
Host
Goat
Theoretical MW (kDa)
39.6
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:2000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
WNT3 polyclonal antibody (Cat # PAB7032) (1 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
WNT3 polyclonal antibody (Cat # PAB7032, 2 ug/mL) staining of paraffin embedded human breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Immunocytochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — WNT3
Entrez GeneID
7473Protein Accession#
NP_110380.1Gene Name
WNT3
Gene Alias
INT4, MGC131950, MGC138321, MGC138323
Gene Description
wingless-type MMTV integration site family, member 3
Gene Ontology
HyperlinkGene Summary
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq
Other Designations
WNT-3 proto-oncogene protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Self-organized formation of developing appendages from murine pluripotent stem cells.
Mori S, Sakakura E, Tsunekawa Y, Hagiwara M, Suzuki T, Eiraku M.
Nature Communications 2019 Aug; 10(1):3802.
Application：Added, Recombinant protein.
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Role of WNT10A-Expressing Kidney Fibroblasts in Acute Interstitial Nephritis.
Kuma A, Yamada S, Wang KY, Kitamura N, Yamaguchi T, Iwai Y, Izumi H, Tamura M, Otsuji Y, Kohno K.
PLoS One 2014 Jul; 9(7):e103240.
Application：IHC-P, WB, Human, Monkey, COS1 cells, Kidney.
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Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping.
Schmitt AM, Shi J, Wolf AM, Lu CC, King LA, Zou Y.
Nature 2006 Jan; 439(7072):31.
Application：IS, WB-Ti, Chick, Retinal .
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Self-organized formation of developing appendages from murine pluripotent stem cells.
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