TXNDC5 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TXNDC5.
Immunogen
A synthetic peptide corresponding to human TXNDC5.
Sequence
C-SLHRFVLSQAKDEL
Host
Goat
Theoretical MW (kDa)
47.6, 36.2
Reactivity
Human
Specificity
This antibody is expected to be able to recognize both reported human isoforms, as represented by NP_110437.2, NP_071368.3.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TXNDC5 polyclonal antibody (Cat # PAB6676) staining (0.1 ug/mL) of human placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TXNDC5
Entrez GeneID
81567Protein Accession#
NP_110437.2;NP_071368.3Gene Name
TXNDC5
Gene Alias
ERP46, EndoPDI, Hcc-2, MGC3178, UNQ364
Gene Description
thioredoxin domain containing 5
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000016005|endothelial protein disulphide isomerase|thioredoxin related protein
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Interactome
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Disease
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Publication Reference
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EndoPDI, a novel protein-disulfide isomerase-like protein that is preferentially expressed in endothelial cells acts as a stress survival factor.
Sullivan DC, Huminiecki L, Moore JW, Boyle JJ, Poulsom R, Creamer D, Barker J, Bicknell R.
The Journal of Biological Chemistry 2003 Nov; 278(47):47079.
Application:WB, Human, Human dermal microvascular endothelial cells.
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EndoPDI, a novel protein-disulfide isomerase-like protein that is preferentially expressed in endothelial cells acts as a stress survival factor.
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