ACTA2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
ACTA2 polyclonal antibody (Cat # PAB6668) (1 ug/mL) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot (Cell lysate)
ACTA2 polyclonal antibody (Cat # PAB6668) (1 ug/mL) staining of HeLa (1) and NIH3T3 (2) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ACTA2.
Immunogen
A synthetic peptide corresponding to N-terminus of human ACTA2.
Sequence
EEEDSTALVC
Host
Goat
Theoretical MW (kDa)
42
Reactivity
Human, Mouse
Specificity
Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.3 - 1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ACTA2 polyclonal antibody (Cat # PAB6668) (1 ug/mL) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Western Blot (Cell lysate)
ACTA2 polyclonal antibody (Cat # PAB6668) (1 ug/mL) staining of HeLa (1) and NIH3T3 (2) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACTA2
Entrez GeneID
59Protein Accession#
NP_001604.1;NP_001135417.1Gene Name
ACTA2
Gene Alias
AAT6, ACTSA
Gene Description
actin, alpha 2, smooth muscle, aorta
Omim ID
102620Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000020042|alpha 2 actin|alpha-cardiac actin|growth-inhibiting gene 46
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Interactome
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Pathway
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Disease
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Publication Reference
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Novel HLA-G-binding leukocyte immunoglobulin-like receptor (LILR) expression patterns in human placentas and umbilical cords.
McIntire RH, Sifers T, Platt JS, Ganacias KG, Langat DK, Hunt JS.
Placenta 2008 Jun; 29(7):631.
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Novel HLA-G-binding leukocyte immunoglobulin-like receptor (LILR) expression patterns in human placentas and umbilical cords.
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