IFT74 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
IFT74 polyclonal antibody (Cat # PAB6611) staining (0.3 ug/mL) of 293 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IFT74 polyclonal antibody (Cat # PAB6611, 5 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data were kindly provided by Dr. Bryan Traynor.
Immunofluorescence
A) IFT74 polyclonal antibody (Cat # PAB6611) (2.5 mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor. See Application Notes!)
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of IFT74.
Immunogen
A synthetic peptide corresponding to C-terminus of human IFT74.
Sequence
C-KTIVDALHSTSGN
Host
Goat
Theoretical MW (kDa)
69.2, 51.4
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
IFT74 polyclonal antibody (Cat # PAB6611) staining (0.3 ug/mL) of 293 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IFT74 polyclonal antibody (Cat # PAB6611, 5 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data were kindly provided by Dr. Bryan Traynor.Immunofluorescence
A) IFT74 polyclonal antibody (Cat # PAB6611) (2.5 mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor. See Application Notes!)Enzyme-linked Immunoabsorbent Assay
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Gene Info — IFT74
Entrez GeneID
80173Protein Accession#
NP_079379.1;AAK77221.1Gene Name
IFT74
Gene Alias
CCDC2, CMG-1, CMG1, FLJ22621, MGC111562
Gene Description
intraflagellar transport 74 homolog (Chlamydomonas)
Omim ID
608040Gene Ontology
HyperlinkOther Designations
OTTHUMP00000022772|capillary morphogenesis protein 1|coiled-coil domain containing 2
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Interactome
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Publication Reference
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Constitutively-active FGFR3 disrupts primary cilium length and IFT20 trafficking in various chondrocyte models of achondroplasia.
Martin L, Kaci N, Estibals V, Goudin N, Garfa-Traore M, Benoist-Lasselin C, Dambroise E, Legeai-Mallet L.
Human Molecular Genetics 2017 Oct; 27(1):1.
Application：ICC, IF, Mouse, Cultured chondrocytes.
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Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD.
Momeni P, Schymick J, Jain S, Cookson MR, Cairns NJ, Greggio E, Greenway MJ, Berger S, Pickering-Brown S, Chio A, Fung HC, Holtzman DM, Huey ED, Wassermann EM, Adamson J, Hutton ML, Rogaeva E, St George-Hyslop P, Rothstein JD, Hardiman O, Grafman J, Singleton A, Hardy J, Traynor BJ.
BMC Neurology 2006 Dec; 6:44.
Application：IF, WB-Ti, Human, Rat, Human brain soluble extracts, Rat cortical neurons.
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Constitutively-active FGFR3 disrupts primary cilium length and IFT20 trafficking in various chondrocyte models of achondroplasia.
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