KPNA3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
KPNA3 polyclonal antibody (Cat # PAB6560) (0.1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
KPNA3 polyclonal antibody (Cat # PAB6560) (0.1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
KPNA3 polyclonal antibody (Cat # PAB6560, 5 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of KPNA3.
Immunogen
A synthetic peptide corresponding to C-terminus of human KPNA3.
Sequence
C-DPTANLQTKEFNF
Host
Goat
Theoretical MW (kDa)
57.8
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
KPNA3 polyclonal antibody (Cat # PAB6560) (0.1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
KPNA3 polyclonal antibody (Cat # PAB6560) (0.1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
KPNA3 polyclonal antibody (Cat # PAB6560, 5 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — KPNA3
Entrez GeneID
3839Protein Accession#
NP_002258.2Gene Name
KPNA3
Gene Alias
IPOA4, SRP1, SRP1gamma, SRP4, hSRP1
Gene Description
karyopherin alpha 3 (importin alpha 4)
Omim ID
601892Gene Ontology
HyperlinkGene Summary
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. [provided by RefSeq
Other Designations
OTTHUMP00000018426|importin alpha 4|importin alpha-3|importin-alpha-Q2|karyopherin alpha 3
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Interactome
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Disease
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Publication Reference
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Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family.
Kohler M, Ansieau S, Prehn S, Leutz A, Haller H, Hartmann E.
FEBS Letters 1997 Nov; 417(1):104.
Application：WB-Ce, WB-Ti, Human, C2C12, COS-7, ECV 304, HEK 293, HeLa, HepG2, HUVEC, Jurkat, MEL, MRC5, MT1, Raji, T23/96, U-937 cells, Brains, Hearts, Lungs, Kidneys, Ovaries, Spleens, Testes.
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Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family.
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