ACAP2 polyclonal antibody
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More Files
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of ACAP2.
Immunogen
A synthetic peptide corresponding to human ACAP2.
Sequence
C-EKLNRFQQDSQKF
Host
Goat
Theoretical MW (kDa)
88
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ACAP2 polyclonal antibody (Cat # PAB6494) staining (1 ug/mL) of MOLT 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ACAP2 polyclonal antibody (Cat # PAB6494, 5 ug/mL) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACAP2
Entrez GeneID
23527Protein Accession#
NP_036419Gene Name
ACAP2
Gene Alias
CENTB2, CNT-B2, KIAA0041
Gene Description
ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Omim ID
607766Gene Ontology
HyperlinkGene Summary
O
Other Designations
Arf GAP with coiled coil, ANK repeat and PH domains 2|centaurin, beta 2
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Interactomes
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Pathways
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Publication Reference
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ACAPs are arf6 GTPase-activating proteins that function in the cell periphery.
Jackson TR, Brown FD, Nie Z, Miura K, Foroni L, Sun J, Hsu VW, Donaldson JG, Randazzo PA.
The Journal of Cell Biology 2000 Oct; 151(3):627.
Application:IF, WB-Ce, Human, Mouse, HeLa, Jurkat, C1R, JY, Daudi, B, HEK 293, NIH 3T3 cells, Monocytes.
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ACAPs are arf6 GTPase-activating proteins that function in the cell periphery.
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