ACAP1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ACAP1.
Immunogen
A synthetic peptide corresponding to human ACAP1.
Sequence
C-EKLSRRSHDLHTL
Host
Goat
Theoretical MW (kDa)
81.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ACAP1 polyclonal antibody (Cat # PAB6493) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACAP1
Entrez GeneID
9744Protein Accession#
NP_055531Gene Name
ACAP1
Gene Alias
CENTB1, KIAA0050
Gene Description
ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Omim ID
607763Gene Ontology
HyperlinkGene Summary
O
Other Designations
Arf GAP with coiled coil, ANK repeat and PH domains 1|centaurin beta1|centaurin, beta 1
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Interactome
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Pathway
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Disease
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Publication Reference
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ACAPs are arf6 GTPase-activating proteins that function in the cell periphery.
Jackson TR, Brown FD, Nie Z, Miura K, Foroni L, Sun J, Hsu VW, Donaldson JG, Randazzo PA.
The Journal of Cell Biology 2000 Oct; 151(3):627.
Application:WB-Ce, Human, Mouse, HeLa, Jurkat, C1R, JY, Daudi, B, HEK 293, NIH 3T3 cells, Monocytes.
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ACAPs are arf6 GTPase-activating proteins that function in the cell periphery.
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