COPS2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of COPS2.
Immunogen
A synthetic peptide corresponding to human COPS2.
Sequence
C-LNSLNQAVVSKLA
Host
Goat
Theoretical MW (kDa)
51.6, 52.4
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
COPS2 polyclonal antibody (Cat # PAB6396) staining (1 ug/mL) of NIH/3T3 lysates (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
In paraffin embedded human thyroid gland shows nclear and cytoplasm staining in activated epithelial cells. COPS2 polyclonal antibody (Cat # PAB6396) recommended concentration, 3-5 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — COPS2
Entrez GeneID
9318Protein Accession#
NP_004227.1Gene Name
COPS2
Gene Alias
ALIEN, CSN2, SGN2, TRIP15
Gene Description
COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
Omim ID
604508Gene Ontology
HyperlinkOther Designations
COP9 constitutive photomorphogenic homolog subunit 2|COP9 signalosome complex subunit 2|JAB1-containing signalosome subunit 2|thyroid receptor interacting protein 15
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Interactome
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Publication Reference
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Various members of the E2F transcription factor family interact in vivo with the corepressor alien.
Escher N, Kob R, Tenbaum SP, Eisold M, Baniahmad A, von Eggeling F, Melle C.
Journal of Proteome Research 2007 Mar; 6(3):1158.
Application:IP, WB, Human, U2OS cells.
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Various members of the E2F transcription factor family interact in vivo with the corepressor alien.
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