ARIH1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
ARIH1 polyclonal antibody (Cat # PAB6322) staining (0.5 ug/mL) of U-937 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ARIH1.
Immunogen
A synthetic peptide corresponding to human ARIH1.
Sequence
C-HEGYEKDLWEYIED
Host
Goat
Theoretical MW (kDa)
64
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.2-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ARIH1 polyclonal antibody (Cat # PAB6322) staining (0.5 ug/mL) of U-937 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ARIH1
Entrez GeneID
25820Protein Accession#
NP_005735Gene Name
ARIH1
Gene Alias
ARI, DKFZp686O13120, FLJ20329, FLJ93118, HARI, HHARI, UBCH7BP
Gene Description
ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila)
Omim ID
605624Gene Ontology
HyperlinkGene Summary
ubiquitin-conjugating enzyme E2 binding protein
Other Designations
ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1|ariadne, Drosophila, homolog of
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Interactome
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Publication Reference
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The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1.
Moynihan TP, Ardley HC, Nuber U, Rose SA, Jones PF, Markham AF, Scheffner M, Robinson PA.
The Journal of Biological Chemistry 1999 Oct; 274(43):30963.
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The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1.
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