MRPL3 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
MRPL3 polyclonal antibody (Cat # PAB6274) staining (1 ug/mL) of HepG2 cell lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MRPL3.
Immunogen
A synthetic peptide corresponding to human MRPL3.
Sequence
NVCQPGAPSITFA
Host
Goat
Theoretical MW (kDa)
38.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
MRPL3 polyclonal antibody (Cat # PAB6274) staining (1 ug/mL) of HepG2 cell lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — MRPL3
Entrez GeneID
11222Protein Accession#
NP_009139.1Gene Name
MRPL3
Gene Alias
MRL3, RPML3
Gene Description
mitochondrial ribosomal protein L3
Omim ID
607118Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L3P ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 13q. [provided by RefSeq
Other Designations
mitochondrial 60S ribosomal protein L3|ribosomal protein, mitochondrial, L3
-
Interactome
-
Publication Reference
-
Cloning and characterization of a human ribosomal protein gene with enhanced expression in fetal and neoplastic cells.
Ou JH, Yen TS, Wang YF, Kam WK, Rutter WJ.
Nucleic Acids Research 1987 Nov; 15(21):8919.
-
Cloning and characterization of a human ribosomal protein gene with enhanced expression in fetal and neoplastic cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com