RPS27 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
RPS27 polyclonal antibody (Cat # PAB6240) staining (0.03 ug/mL) of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RPS27.
Immunogen
A synthetic peptide corresponding to human RPS27.
Sequence
PLAKDLLHPSPEE-C
Host
Goat
Theoretical MW (kDa)
9.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.05-0.2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
RPS27 polyclonal antibody (Cat # PAB6240) staining (0.03 ug/mL) of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPS27
Entrez GeneID
6232Protein Accession#
NP_001021.1Gene Name
RPS27
Gene Alias
MPS-1, MPS1
Gene Description
ribosomal protein S27
Omim ID
603702Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
40S ribosomal protein S27 (metallopan-stimulin 1)|OTTHUMP00000034760|metallopanstimulin 1
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Interactome
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Pathway
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Publication Reference
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A growth factor-inducible gene encodes a novel nuclear protein with zinc finger structure.
Fernandez-Pol JA, Klos DJ, Hamilton PD.
The Journal of Biological Chemistry 1993 Oct; 268(28):21198.
Application：IF, Human, MDA-468, WI-38 cells.
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A growth factor-inducible gene encodes a novel nuclear protein with zinc finger structure.
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