FLOT2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
FLOT2 polyclonal antibody (Cat # PAB6175) staining (0.1 ug/mL) of human brain (cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FLOT2.
Immunogen
A synthetic peptide corresponding to human FLOT2.
Sequence
C-SKIPLIKKATGVQV
Host
Goat
Theoretical MW (kDa)
47.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
FLOT2 polyclonal antibody (Cat # PAB6175) staining (0.1 ug/mL) of human brain (cerebellum) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FLOT2
Entrez GeneID
2319Protein Accession#
NP_004466.2Gene Name
FLOT2
Gene Alias
ECS-1, ECS1, ESA, ESA1, M17S1
Gene Description
flotillin 2
Omim ID
131560Gene Ontology
HyperlinkGene Summary
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq
Other Designations
Flotillin 2 (epidermal surface antigen 1)|membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1)
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Interactome
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Pathway
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Publication Reference
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EFR3A: a new raft domain organizing protein?
Magdalena Trybus, Anita Hryniewicz-Jankowska, Karolina Wójtowicz, Tomasz Trombik, Aleksander Czogalla, Aleksander F Sikorski.
Cellular & Molecular Biology Letters 2023 Oct; 28(1):86.
Application：WB, Co-IP, Human, HeLa cells, Recombinant proteins.
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Asymmetric localization of flotillins/reggies in preassembled platforms confers inherent polarity to hematopoietic cells.
Rajendran L, Masilamani M, Solomon S, Tikkanen R, Stuermer CA, Plattner H, Illges H.
PNAS 2003 Jul; 100(14):8241.
Application：IF, WB, Human, Jurkat, U937 cells.
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EFR3A: a new raft domain organizing protein?
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