TFEB polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TFEB.
Immunogen
A synthetic peptide corresponding to N-terminus of human TFEB.
Sequence
ASRIGLRMQLMRE-C
Host
Goat
Theoretical MW (kDa)
52.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Tissue lysate)
TFEB polyclonal antibody (Cat # PAB6161)(0.5 ug/mL) staining of human thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Enzyme-linked Immunoabsorbent Assay
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Gene Info — TFEB
Entrez GeneID
7942Protein Accession#
NP_009093.1Gene Name
TFEB
Gene Alias
AlphaTFEB, TCFEB, bHLHe35
Gene Description
transcription factor EB
Omim ID
600744Gene Ontology
HyperlinkOther Designations
OTTHUMP00000043540|OTTHUMP00000043542|OTTHUMP00000071327|T-cell transcription factor EB
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Interactome
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Publication Reference
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Renal carcinoma-associated transcription factors TFE3 and TFEB are leukemia inhibitory factor-responsive transcription activators of E-cadherin.
Huan C, Sashital D, Hailemariam T, Kelly ML, Roman CA.
The Journal of Biological Chemistry 2005 Aug; 280(34):30225.
Application:ChIP, WB-Ce, WB-Tr, Human, Mouse, 3T3, mIMCD-3, MEF, MEF TFE3-/-, HEK293 cells.
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Renal carcinoma-associated transcription factors TFE3 and TFEB are leukemia inhibitory factor-responsive transcription activators of E-cadherin.
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