MTR polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MTR.
Immunogen
A synthetic peptide corresponding to human MTR.
Sequence
C-VEKWLGPILGYDTD
Host
Goat
Theoretical MW (kDa)
139
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(3-5 ug/mL)
ELISA (1:32000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
MTR polyclonal antibody (Cat # PAB6022) staining (2 ug/mL) of HeLa lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human adrenal gland with MTR polyclonal antibody (Cat # PAB6022) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — MTR
Entrez GeneID
4548Protein Accession#
NP_000245Gene Name
MTR
Gene Alias
FLJ33168, FLJ43216, FLJ45386, MS
Gene Description
5-methyltetrahydrofolate-homocysteine methyltransferase
Gene Ontology
HyperlinkGene Summary
MTR encodes the enzyme 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. [provided by RefSeq
Other Designations
5-methyltetrahydrofolate-homocysteine methyltransferase 1|cobalamin-dependent methionine synthase|methionine synthase|vitamin-B12 dependent methionine synthase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism.
Martinez-Vega R, Garrido F, Partearroyo T, Cediel R, Zeisel SH, Martinez-Alvarez C, Varela-Moreiras G, Varela-Nieto I, Pajares MA.
FASEB Journal 2015 Feb; 29(2):418.
Application:WB, Mouse, Cochlear.
-
Analysis of subcellular [57Co] cobalamin distribution in SH-SY5Y neurons and brain tissue.
Zhao H, Ruberu K, Li H, Garner B.
Journal of Neuroscience Methods 2013 Jul; 217(1-2):67.
Application:WB, Human, Neuroblastoma, Fibrosarcoma cells.
-
Human methionine synthase. cDNA cloning, gene localization, and expression.
Chen LH, Liu ML, Hwang HY, Chen LS, Korenberg J, Shane B.
The Journal of Biological Chemistry 1997 Feb; 272(6):3628.
-
Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com