AKT1 (phospho S473) polyclonal antibody
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More Files
- More Functions
Dot Blot (Peptide)
Dot blot analysis of AKT1 (phospho S473) polyclonal antibody (Cat # PAB4918) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S473 of human AKT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot blot analysis of AKT1 (phospho S473) polyclonal antibody (Cat # PAB4918) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL. -
Gene Info — AKT1
Entrez GeneID
207Protein Accession#
NP_001014431;P31749Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.
Powell DJ, Hajduch E, Kular G, Hundal HS.
Molecular and Cellular Biology 2003 Nov; 23(21):7794.
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Increase of AKT/PKB expression correlates with gleason pattern in human prostate cancer.
Liao Y, Grobholz R, Abel U, Trojan L, Michel MS, Angel P, Mayer D.
International Journal of Cancer 2003 Nov; 107(4):676.
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Akt activation disrupts mammary acinar architecture and enhances proliferation in an mTOR-dependent manner.
Debnath J, Walker SJ, Brugge JS.
Journal of Cellular Biology 2003 Oct; 163(2):315.
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Ceramide disables 3-phosphoinositide binding to the pleckstrin homology domain of protein kinase B (PKB)/Akt by a PKCzeta-dependent mechanism.
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