CLDN1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CLDN1 polyclonal antibody (Cat # PAB4903) in Jurkat cell lysate (35 ug/lane). CLDN1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with CLDN1 polyclonal antibody (Cat # PAB4903) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CLDN1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to the Loop1 region of human CLDN1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CLDN1 polyclonal antibody (Cat # PAB4903) in Jurkat cell lysate (35 ug/lane). CLDN1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with CLDN1 polyclonal antibody (Cat # PAB4903) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CLDN1
Entrez GeneID
9076Protein Accession#
NP_066924;O95832Gene Name
CLDN1
Gene Alias
CLD1, ILVASC, SEMP1
Gene Description
claudin 1
Gene Ontology
HyperlinkGene Summary
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq
Other Designations
senescence-associated epithelial membrane protein 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Selective up-regulation of claudin-1 and claudin-2 in colorectal cancer.
Kinugasa T, Huo Q, Higashi D, Shibaguchi H, Kuroki M, Tanaka T, Futami K, Yamashita Y, Hachimine K, Maekawa S, Nabeshima K, Iwasaki H, Kuroki M.
Anticancer Research 2007 Nov; 27(6A):3729.
Application：IHC-P, Human, Human colorectal Cancer.
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Decreased expression of claudin-1 correlates with recurrence status in breast cancer.
Morohashi S, Kusumi T, Sato F, Odagiri H, Chiba H, Yoshihara S, Hakamada K, Sasaki M, Kijima H.
International Journal of Molecular Medicine 2007 Aug; 20(2):139.
Application：IHC-P, Human, Human breast cancer.
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Claudin-1 and claudin-5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas.
Paschoud S, Bongiovanni M, Pache JC, Citi S.
Modern Pathology 2007 Sep; 20(9):947.
Application：IHC-P, Human, Human lung cancer.
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Selective up-regulation of claudin-1 and claudin-2 in colorectal cancer.
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