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Last updated: 2014/10/20
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CD14 polyclonal antibody

  • Catalog # : PAB4881
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CD14.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CD14.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:50-100)
    Immunohistochemistry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of CD14 (arrow) using rabbit CD14 polyclonal antibody (Cat # PAB4881). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 polyclonal antibody (Cat # PAB4881) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 929
  • Protein Accession#:
  • NP_000582;P08571
  • Gene Name:
  • CD14
  • Gene Alias:
  • -
  • Gene Description:
  • CD14 molecule
  • Gene Summary:
  • CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq
  • Other Designations:
  • CD14 antigen,monocyte differentiation antigen CD14
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