CD14 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of CD14 (arrow) using rabbit CD14 polyclonal antibody (Cat # PAB4880). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 polyclonal antibody (Cat # PAB4880) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CD14.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CD14.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of CD14 (arrow) using rabbit CD14 polyclonal antibody (Cat # PAB4880). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 polyclonal antibody (Cat # PAB4880) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CD14
Entrez GeneID
929Protein Accession#
NP_000582;P08571Gene Name
CD14
Gene Alias
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Gene Description
CD14 molecule
Omim ID
158120Gene Ontology
HyperlinkGene Summary
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq
Other Designations
CD14 antigen|monocyte differentiation antigen CD14
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Interactome
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Pathway
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Disease
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Publication Reference
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Local expression of interleukin-10 and mCD14 in relation to the -1087 IL-10 and -159 CD14 gene polymorphisms in chronic periodontitis.
Donati M, Liljenberg B, Padyukov L, Berglundh T.
Journal of Periodontology 2008 Mar; 79(3):517.
Application：IHC-Fr, Human, patients with chronic periodontitis.
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Clinical relevance of TLR2, TLR4, CD14 and FcgammaRIIA gene polymorphisms in Streptococcus pneumoniae infection.
Yuan FF, Marks K, Wong M, Watson S, de Leon E, McIntyre PB, Sullivan JS.
Immunology and Cell Biology 2008 Mar; 86(3):268.
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Mouse and human CD14 (myeloid cell-specific leucine-rich glycoprotein) primary structure deduced from cDNA clones.
Setoguchi M, Nasu N, Yoshida S, Higuchi Y, Akizuki S, Yamamoto S.
Biochimica et Biophysica Acta 1989 Jul; 1008(2):213.
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Local expression of interleukin-10 and mCD14 in relation to the -1087 IL-10 and -159 CD14 gene polymorphisms in chronic periodontitis.
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