EDA polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of EDA polyclonal antibody (Cat # PAB4866) in HeLa cell lysate (35 ug/lane). EDA (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with EDA polyclonal antibody (Cat # PAB4866) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Flow Cytometry
Flow cytometric analysis of HeLa cells using EDA polyclonal antibody (Cat # PAB4866)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EDA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EDA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of EDA polyclonal antibody (Cat # PAB4866) in HeLa cell lysate (35 ug/lane). EDA (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with EDA polyclonal antibody (Cat # PAB4866) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using EDA polyclonal antibody (Cat # PAB4866)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — EDA
Entrez GeneID
1896Protein Accession#
NP_001390;Q92838Gene Name
EDA
Gene Alias
ED1, ED1-A1, ED1-A2, EDA1, EDA2, HED, XHED, XLHED
Gene Description
ectodysplasin A
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000023461|OTTHUMP00000023463|X-linked anhidroitic ectodermal dysplasia protein|ectodermal dysplasia 1, anhidrotic|ectodermal dysplasia, anhidrotic (hypohydrotic)|ectodysplasin-A
-
Interactome
-
Pathway
-
Publication Reference
-
A novel 4-bp insertion mutation in EDA1 gene in a Pakistani family with X-linked hypohidrotic ectodermal dysplasia.
Muhammad Tariq, Naveed Wasif, Muhammad Ayub, Wasim Ahmad.
European Journal of Dermatology 2007 May; 17(3):209.
-
A novel Gln358Glu mutation in ectodysplasin A associated with X-linked dominant incisor hypodontia.
Tarpey P, Pemberton TJ, Stockton DW, Das P, Ninis V, Edkins S, Andrew Futreal P, Wooster R, Kamath S, Nayak R, Stratton MR, Patel PI.
American Journal of Medical Genetics. Part A 2007 Feb; 143(4):390.
-
A novel 4-bp insertion mutation in EDA1 gene in a Pakistani family with X-linked hypohidrotic ectodermal dysplasia.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com