HIRA polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HIRA polyclonal antibody (Cat # PAB4861) in HL-60 cell lysate (35 ug/lane). HIRA (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HIRA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human HIRA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of HIRA polyclonal antibody (Cat # PAB4861) in HL-60 cell lysate (35 ug/lane). HIRA (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — HIRA
Entrez GeneID
7290Protein Accession#
NP_003316;P54198Gene Name
HIRA
Gene Alias
DGCR1, TUP1, TUPLE1
Gene Description
HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
Omim ID
600237Gene Ontology
HyperlinkGene Summary
This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq
Other Designations
DiGeorge critical region gene 1|HIR histone cell cycle regulation defective homolog A
-
Interactome
-
Disease
-
Publication Reference
-
Molecular dissection of formation of senescence-associated heterochromatin foci.
Zhang R, Chen W, Adams PD.
Molecular and Cellular Biology 2007 Mar; 27(6):2343.
Application：IF, Human, WI-38 cells .
-
Structure of a human ASF1a-HIRA complex and insights into specificity of histone chaperone complex assembly.
Tang Y, Poustovoitov MV, Zhao K, Garfinkel M, Canutescu A, Dunbrack R, Adams PD, Marmorstein R.
Nature Structural & Molecular Biology 2006 Oct; 13(10):921.
-
Molecular dissection of formation of senescence-associated heterochromatin foci.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com