MMP8 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MMP8.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human MMP8.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of MMP8 polyclonal antibody (Cat # PAB4797) in mouse liver tissue lysate (35 ug/lane). MMP8 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MMP8 polyclonal antibody (Cat # PAB4797) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MMP8
Entrez GeneID
4317Protein Accession#
MM8_HUMANGene Name
MMP8
Gene Alias
CLG1, HNC, PMNL-CL
Gene Description
matrix metallopeptidase 8 (neutrophil collagenase)
Omim ID
120355Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq
Other Designations
PMNL collagenase|matrix metalloproteinase 8|matrix metalloproteinase 8 (neutrophil collagenase)|neutrophil collagenase
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Interactome
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Disease
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Publication Reference
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Carboxyterminal cleavage of the chemokines MIG and IP-10 by gelatinase B and neutrophil collagenase.
Van den Steen PE, Husson SJ, Proost P, Van Damme J, Opdenakker G.
Biochemical and Biophysical Research Communications 2003 Oct; 10(3):889.
Application:WB, Recombinant protein.
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Neutrophil collagenase (MMP-8) is expressed during early development in neural crest cells as well as in adult melanoma cells.
Giambernardi TA, Sakaguchi AY, Gluhak J, Pavlin D, Troyer DA, Das G, Rodeck U, Klebe RJ.
Matrix Biology 2001 Dec; 20(8):577.
Application:IHC-P, Human, Mouse, Human melanoma tumors, Mouse embryos.
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Matrix metalloproteinases.
Nagase H, Woessner JF Jr.
The Journal of Biological Chemistry 1999 Jul; 274(31):21491.
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Carboxyterminal cleavage of the chemokines MIG and IP-10 by gelatinase B and neutrophil collagenase.
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