GREM1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of mouse liver cell lysate using GREM1 polyclonal antibody (Cat # PAB4716). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot (Cell lysate)
Western blot analysis of CEM cell line lysates (35 ug/lane) using GREM1 polyclonal antibody (Cat # PAB4716).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREM1 polyclonal antibody (Cat # PAB4716) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of GREM1 polyclonal antibody (Cat # PAB4716) was 1 : 50. Detection method was ABC system or Vectastaint Elite VECTOR. Data and protocol courtesy of Dr. Maria E. Burgos of Universidad Austral de Chile.
Immunohistochemistry
ISH and IMH demonstrating GREM1 mRNA and GREM1 protein in crescentic PECs and tubular epithelial cells of patients with pauci-immune crescentic GN. In comparison with normal renal tissue in which there is no expression of GREM1 mRNA (C), proliferating PECs of glomerular crescents show a strong expression of GREM1 mRNA by ISH (A, D) ; and GREM1 protein expression by IMH (B) ; immune competent infiltrating interstitial cells are also strongly positive for GREM1 staining (E) ; and CTGF was also expressed in these glomerular crescentic cells (F).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GREM1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human GREM1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Saturated Ammonium Sulfate (SAS) precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse liver cell lysate using GREM1 polyclonal antibody (Cat # PAB4716). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Western Blot (Cell lysate)
Western blot analysis of CEM cell line lysates (35 ug/lane) using GREM1 polyclonal antibody (Cat # PAB4716).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREM1 polyclonal antibody (Cat # PAB4716) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of GREM1 polyclonal antibody (Cat # PAB4716) was 1 : 50. Detection method was ABC system or Vectastaint Elite VECTOR. Data and protocol courtesy of Dr. Maria E. Burgos of Universidad Austral de Chile.Immunohistochemistry
ISH and IMH demonstrating GREM1 mRNA and GREM1 protein in crescentic PECs and tubular epithelial cells of patients with pauci-immune crescentic GN. In comparison with normal renal tissue in which there is no expression of GREM1 mRNA (C), proliferating PECs of glomerular crescents show a strong expression of GREM1 mRNA by ISH (A, D) ; and GREM1 protein expression by IMH (B) ; immune competent infiltrating interstitial cells are also strongly positive for GREM1 staining (E) ; and CTGF was also expressed in these glomerular crescentic cells (F).Enzyme-linked Immunoabsorbent Assay
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Gene Info — GREM1
Entrez GeneID
26585Protein Accession#
NP_037504;O70326Gene Name
GREM1
Gene Alias
CKTSF1B1, DAND2, DRM, GREMLIN, IHG-2, MGC126660, PIG2
Gene Description
gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis)
Omim ID
603054Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth. [provided by RefSeq
Other Designations
cysteine knot superfamily 1, BMP antagonist 1|down-regulated in Mos-transformed cells|gremlin 1-like protein|gremlin-1|increased in high glucose-2|proliferation-inducing gene 2
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Interactome
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Disease
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Publication Reference
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Expression of gremlin, a bone morphogenetic protein antagonist,is associated with vascular calcification in uraemia.
Jara A, Chacon C, Burgos ME, Droguett A, Valdivieso A, Ortiz M, Troncoso P, Mezzano S.
Nephrology, Dialysis, Transplantation 2008 Nov; 24(4):1121.
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Expression of gremlin, a bone morphogenetic protein antagonist, in glomerular crescents of pauci-immune glomerulonephritis.
Mezzano S, Droguett A, Burgos ME, Aros C, Ardiles L, Flores C, Carpio D, Carvajal G, Ruiz-Ortega M, Egido J.
Nephrology, Dialysis, Transplantation 2007 Apr; 22(7):1882.
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Effects of TGF-beta2, BMP-4, and gremlin in the trabecular meshwork: implications for glaucoma.
Wordinger RJ, Fleenor DL, Hellberg PE, Pang IH, Tovar TO, Zode GS, Fuller JA, Clark AF.
Investigative Ophthalmology & Visual Science 2007 Mar; 48(3):1191.
Application：WB, Human, Human glaucoma.
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Expression of gremlin, a bone morphogenetic protein antagonist,is associated with vascular calcification in uraemia.
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