BACE2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The BACE2 polyclonal antibody (Cat # PAB4706) is used in Western blot to detect BACE2 in HL-60 cell lysate.
Flow Cytometry
Flow cytometric analysis of HL-60 cells using BACE2 polyclonal antibody (Cat # PAB4706)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BACE2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human BACE2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The BACE2 polyclonal antibody (Cat # PAB4706) is used in Western blot to detect BACE2 in HL-60 cell lysate.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HL-60 cells using BACE2 polyclonal antibody (Cat # PAB4706)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — BACE2
Entrez GeneID
25825Protein Accession#
Q9Y5Z0Gene Name
BACE2
Gene Alias
AEPLC, ALP56, ASP1, ASP21, BAE2, CDA13, CEAP1, DRAP
Gene Description
beta-site APP-cleaving enzyme 2
Omim ID
605668Gene Ontology
HyperlinkGene Summary
Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease and a frequent complication of Down syndrome. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein by 2 proteases, one of which is the protein encoded by this gene. This gene localizes to the 'Down critical region' of chromosome 21. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease. Three transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
56 kDa aspartic-like protease|Down syndrome region aspartic protease|aspartyl protease 1|beta secretase 2|beta-site amyloid beta A4 precursor protein-cleaving enzyme 2|memapsin-1|membrane-associated aspartic protease 1|transmembrane aspartic proteinase As
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Interactome
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Disease
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Publication Reference
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The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A.
Genome Research 2003 Sep; 13(10):2265.
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BACE-2 is overexpressed in Down's syndrome.
Barbiero L, Benussi L, Ghidoni R, Alberici A, Russo C, Schettini G, Pagano SF, Parati EA, Mazzoli F, Nicosia F, Signorini S, Feudatari E, Binetti G.
Experimental Neurology 2003 Aug; 182(2):335.
Application：WB-Ce, WB-Ti, Human, Fibroblast, Embryonic neural stem cells, Brain.
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Antagonistic effects of beta-site amyloid precursor protein-cleaving enzymes 1 and 2 on beta-amyloid peptide production in cells.
Basi G, Frigon N, Barbour R, Doan T, Gordon G, McConlogue L, Sinha S, Zeller M.
The Journal of Biological Chemistry 2003 Aug; 278(34):31512.
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The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
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