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Last updated: 2014/4/21
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BIOtech
BULK-new

ACE2 polyclonal antibody

  • Catalog # : PAB4685
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ACE2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to internal region of human ACE2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Immunohistochemistry (1:50-100)
    Western Blot (1:50-200)
    Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of HL-60 cell lysate (35 ug/lane) with ACE2 polyclonal antibody (Cat # PAB4685).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with ACE2 polyclonal antibody (Cat # PAB4685) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HL-60 cells using ACE2 polyclonal antibody (Cat # PAB4685)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_068576;Q9BYF1
  • Gene Name:
  • ACE2
  • Gene Alias:
  • ACEH,DKFZp434A014
  • Gene Description:
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • Gene Summary:
  • The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq
  • Other Designations:
  • ACE-related carboxypeptidase,OTTHUMP00000022963,angiotensin I converting enzyme 2,angiotensin converting enzyme-like protein
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