PARP1 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PARP1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PARP1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of PARP1 (arrow) using rabbit PARP1 polyclonal antibody (Cat # PAB4469). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PARP1 gene (Lane 2) (Origene Technologies).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PARP1
Entrez GeneID
142Protein Accession#
NP_001609;P09874Gene Name
PARP1
Gene Alias
ADPRT, ADPRT1, PARP, PARP-1, PPOL, pADPRT-1
Gene Description
poly (ADP-ribose) polymerase 1
Omim ID
173870Gene Ontology
HyperlinkGene Summary
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq
Other Designations
ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|OTTHUMP00000035663|poly (ADP-ribose) polymerase family, member 1|poly(ADP-ribose) polymerase|poly(ADP-ribose) synthetase|poly(ADP-ribosyl)transferase
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Domain C of human poly(ADP-ribose) polymerase-1 is important for enzyme activity and contains a novel zinc-ribbon motif.
Tao Z, Gao P, Hoffman DW, Liu HW.
Biochemistry 2008 May; 47(21):5804.
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Ethnic disparities in Americans of European descent versus Americans of African descent related to polymorphic ERCC1, ERCC2, XRCC1, and PARP1.
Gao R, Price DK, Sissung T, Reed E, Figg WD.
Molecular Cancer Therapeutics 2008 May; 7(5):1246.
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The human poly(ADP-ribose) polymerase nuclear localization signal is a bipartite element functionally separate from DNA binding and catalytic activity.
Schreiber V, Molinete M, Boeuf H, de Murcia G, Menissier-de Murcia J.
The EMBO Journal 1992 Sep; 11(9):3263.
Application:IF, Human, HeLa cells.
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Domain C of human poly(ADP-ribose) polymerase-1 is important for enzyme activity and contains a novel zinc-ribbon motif.
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