IL1RL2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of IL1R1 polyclonal antibody (Cat # PAB4455) in HL-60 cell line lysates (35 ug/lane). IL1R1 (arrow) was detected using the purified IL1R1 polyclonal antibody (Cat # PAB4455) (1 : 60 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with IL1R1 polyclonal antibody (Cat # PAB4455) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IL1RL2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus (E487) of human IL1RL2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of IL1R1 polyclonal antibody (Cat # PAB4455) in HL-60 cell line lysates (35 ug/lane). IL1R1 (arrow) was detected using the purified IL1R1 polyclonal antibody (Cat # PAB4455) (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with IL1R1 polyclonal antibody (Cat # PAB4455) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .Enzyme-linked Immunoabsorbent Assay
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Gene Info — IL1RL2
Entrez GeneID
8808Protein Accession#
NP_003845;Q9HB29Gene Name
IL1RL2
Gene Alias
IL1R-rp2, IL1RRP2
Gene Description
interleukin 1 receptor-like 2
Omim ID
604512Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the interleukin 1 receptor family. An experiment with transient gene expression demonstrated that this receptor was incapable of binding to interleukin 1 alpha and interleukin 1 beta with high affinity. This gene and four other interleukin 1 receptor family genes, including interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 1 (IL1RL1), and interleukin 18 receptor 1 (IL18R1), form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq
Other Designations
interleukin-1 receptor-related protein 2
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Interactome
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Disease
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Publication Reference
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Two novel IL-1 family members, IL-1 delta and IL-1 epsilon, function as an antagonist and agonist of NF-kappa B activation through the orphan IL-1 receptor-related protein 2.
Debets R, Timans JC, Homey B, Zurawski S, Sana TR, Lo S, Wagner J, Edwards G, Clifford T, Menon S, Bazan JF, Kastelein RA.
Journal of Immunology 2001 Aug; 167(3):1440.
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Interleukin-1 receptor cluster: gene organization of IL1R2, IL1R1, IL1RL2 (IL-1Rrp2), IL1RL1 (T1/ST2), and IL18R1 (IL-1Rrp) on human chromosome 2q.
Dale M, Nicklin MJ.
Genomics 1999 Apr; 57(1):177.
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Purification and characterization of the human interleukin-18 receptor.
Torigoe K, Ushio S, Okura T, Kobayashi S, Taniai M, Kunikata T, Murakami T, Sanou O, Kojima H, Fujii M, Ohta T, Ikeda M, Ikegami H, Kurimoto M.
The Journal of Biological Chemistry 1997 Oct; 272(41):25737.
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Two novel IL-1 family members, IL-1 delta and IL-1 epsilon, function as an antagonist and agonist of NF-kappa B activation through the orphan IL-1 receptor-related protein 2.
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