CPT1B polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of CPT1B (arrow) using rabbit CPT1B polyclonal antibody (Cat # PAB4375). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CPT1B gene (Lane 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CPT1B polyclonal antibody (Cat # PAB4375) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CPT1B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CPT1B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-200)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western blot analysis of CPT1B (arrow) using rabbit CPT1B polyclonal antibody (Cat # PAB4375). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CPT1B gene (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CPT1B polyclonal antibody (Cat # PAB4375) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .Enzyme-linked Immunoabsorbent Assay
-
Gene Info — CPT1B
Entrez GeneID
1375Protein Accession#
CPTM_HUMANGene Name
CPT1B
Gene Alias
CPT1-M, KIAA1670, M-CPT1, MCCPT1
Gene Description
carnitine palmitoyltransferase 1B (muscle)
Omim ID
601987Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq
Other Designations
CPTI, muscle|carnitine O-palmitoyltransferase I, mitochondrial muscle isoform|carnitine palmitoyltransferase 1A (muscle)|carnitine palmitoyltransferase 1B
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Leucine-764 near the extreme C-terminal end of carnitine palmitoyltransferase I is important for activity.
Dai J, Zhu H, Woldegiorgis G.
Biochemical and Biophysical Research Communications 2003 Feb; 301(3):758.
Application：WB, Yeast, Yeast cells.
-
Structural and functional genomics of the CPT1B gene for muscle-type carnitine palmitoyltransferase I in mammals.
van der Leij FR, Cox KB, Jackson VN, Huijkman NC, Bartelds B, Kuipers JR, Dijkhuizen T, Terpstra P, Wood PA, Zammit VA, Price NT.
The Journal of Biological Chemistry 2002 Jul; 277(30):26994.
-
Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping.
Hirosawa M, Nagase T, Murahashi Y, Kikuno R, Ohara O.
DNA Research 2001 Feb; 8(1):1.
-
Leucine-764 near the extreme C-terminal end of carnitine palmitoyltransferase I is important for activity.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com