TRIM69 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of TRIM69 polyclonal antibody (Cat # PAB4372) in HL-60 cell line lysates (35 ug/lane). TRIM69 (arrow) was detected using the purified TRIM69 polyclonal antibody (Cat # PAB4372) (1 : 60 dilution).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TRIM69.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human TRIM69.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of TRIM69 polyclonal antibody (Cat # PAB4372) in HL-60 cell line lysates (35 ug/lane). TRIM69 (arrow) was detected using the purified TRIM69 polyclonal antibody (Cat # PAB4372) (1 : 60 dilution).Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRIM69
Entrez GeneID
140691Protein Accession#
NP_542783;Q86WT6Gene Name
TRIM69
Gene Alias
HSD34, RNF36, Trif
Gene Description
tripartite motif-containing 69
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
ring finger protein 36|trimless|tripartite motif protein 69
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Interactome
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Disease
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Publication Reference
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Forced expression of RNF36 induces cell apoptosis.
Shyu HW, Hsu SH, Hsieh-Li HM, Li H.
Experimental Cell Research 2003 Jul; 287(2):301.
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A novel member of the RBCC family, Trif, expressed specifically in the spermatids of mouse testis.
Shyu HW, Hsu SH, Hsieh-Li HM, Li H.
Mechanisms of Development 2001 Oct; 108(1-2):213.
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Forced expression of RNF36 induces cell apoptosis.
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